1.Mainly used as a standard for the determination of octane number, and can also be used as anesthetics, solvents and raw materials for organic synthesis 2.Used as analytical reagent and solvent 3.Extraction solvent 4.As analytical reagents 5.Biochemical studies, protein sequence analysis. Determination of ozone, triglyceride. Pesticide residue analysis. Spectroscopic and fluorescence analysis. Standard fuel for the determination of antiknock properties of gasoline