ZymtanTM DH is an alkaline lipolytic enzyme preparation as a processing aid in degreasing process in leather manufacturing, which is produced by fermentation with a selected strain of a microorganism. ZymtanTM DH is designed to be used to improve fat dispersion and production of waterproof and low-fogging leathers. Systematically hydrolyzing the adipose tissue, the product is most effective at removing lipids from the surface and sebaceous glands during the soaking and/or liming stages, which reduces residual hair and surface grease substantially and improves the uniformity of dyeing and retaining and supports inner softness. The product can support the soaking effect and enhances the penetration of other chemicals leading to an improved layout and area yield. The product can also be applied in an acid process, e.g. for pickled skin or wool-on and fur, or a semi-acid process for wet blue.