Antiseptic hands Bactericidal 99,9 % of bacteria: S. aureus, P. aeruginosa, S. flexneri, B. subtlilis, C. albicans, M. tuberculosis within 30 seconds of exposure and 03 minutes of exposure Ethanol 70% Regular Antiseptic hands: Getting 3 ml of pure hand sanitizer (equivalent to 02 pumps/ 02 squeezes/ 06 sprays) into the hands and rubbing hands according to the Health Ministry's instructions with minimum 30 seconds. Then let your hands dry with air and don't rinse with water. Hand sanitizer before surgery: performing 6 steps, each step getting 3 ml of pure hand sanitizer (equivalent to 02 pumps/ 02 squeezes/ 06 sprays) into the hands. Rubbing hands according to the Ministry of Health's instructions on the procedure of surgical hand hygiene with minimum of 3 minutes