SolGent DiaPlexQâ?¢ Novel Coronavirus (2019-nCoV) Detection Kit CE-IVD Real-Time OneStep RT-PCR based assay system for detection of 2019-nCoV CE-IVD DiaPlexQâ?¢ Novel Coronavirus(2019-nCoV) Detection Kit is CE-IVD reagent. It is screening kit to detect Novel Corona Virus as 2019-nCoV from isolated total RNA. Basic principal of this kit is Real-time PCR method, which is able to detect specific target gene into total RNA. One step RT-PCR contents are progressively apply for one tube RT(Reverse Transcription) reaction and PCR amplification. You can monitoring the nucleic acid amplification result based on real time condition through the amplification plot. The Control Template (2019-nCoV) is provide as positive control to assist the clinical sample data comparison analysis. - Detection target : 2019-nCoV ( COVID-19 ) - Target region : Orf1a, N gene - Detection technology : Real-Time OneStep RT-qPCR - Specimen type : Nasopharyngeal swab, Oropharyngeal swab, Sputum - Compatible instruments : CFX96â?¢ Real-Time PCR System (Bio-Rad), ABI 7500 / 7500 Fast Real-Time PCR System (Applied Biosystems) - PCR running time : ~ 120 mins - Simple & Rapid detection system: OneStep Multiplex RT-qPCR based detection - HotStart PCR: high-specificity - Reliable system: automatic PCR control (not Internal control) - Easy-to-use master mix: just adding template and Primer/Probe Mix - Positive control included (Plasmid)