The one step sulfamethazine test strip is a rapid test to qualitatively detect the presence of sulfamethazine in specimens at the sensitivity of 10ppb. The major advantages of the one step test strip are that results can be obtained within 10 min and that all reagents are included in the test strip. It relatively has many advantages such as lower cost , fewer lable and berif sample preparation than the traditional test methods . Using the one step sulfamethazine test strip, it is possible to detect sulfamethazine below the maximum residue limit in muscle, tissue after a simple sample preparation.