ROC-F-OR003K Melamine ELISA Test Kit
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Melamine in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Melamine in the sample and the coupling antigens pre-coated on the micro-well stripes compete for anti-Melamine antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample has a negative correlation with the Melamine concentration in the sample. This value is compared to the standard curve and the Melamine concentration is subsequently obtained.

2. Technical specifications
Sensitivity: 0.5 ppb
Incubator temperature: 25
Incubator time: 30minï½?15min
Detection limit:
Milk powder 10 ppb
Milk 1 ppb
Feed 50 ppb
Cross-reaction rate:
Melamine 100%
Cyanuric acid 60%
Triazine