ROC-F-OR003K Melamine ELISA Test Kit 1. Principle This test kit is based on the competitive enzyme immunoassay for the detection of Melamine in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Melamine in the sample and the coupling antigens pre-coated on the micro-well stripes compete for anti-Melamine antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample has a negative correlation with the Melamine concentration in the sample. This value is compared to the standard curve and the Melamine concentration is subsequently obtained. 2. Technical specifications Sensitivity: 0.5 ppb Incubator temperature: 25 Incubator time: 30minï½?15min Detection limit: Milk powder 10 ppb Milk 1 ppb Feed 50 ppb Cross-reaction rate: Melamine 100% Cyanuric acid 60% Triazine