4-Hydroxyethylpiperazine Ethane Sulfonic Acid (HEPES) is a zwitterionic buffer, white crystalline powder, soluble in water, it is a weak acid, its pH buffer range is 6.8-8.2, it is important in biochemical industry Biochemical preparations are also considered to be one of the important buffers in the field of biological research. As a veteran company with more than ten years of experience in producing HEPES, Desheng, the confidence we give to customers comes from our advantages in all aspects of products: 1. Advantages of PH range: The suitable culture environment for most cells is pH 7.2-pH 7.4, and the application range of Desheng HEPES buffer just falls between pH 6.8-pH 8.2, which is in line with the characteristics of the pH value needed for cell culture. Purity advantage: Desheng HEPES has a purity of >=99%, good water solubility, stable process, and can control a constant pH range for a long time. According to your application needs, we can provide a full range of product testing and special testing services. Reserve advantages: Desheng has a professional R&D and production team, specializing in the production of HEPES, with a daily output of 1-2 tons, and advanced production technology and equipment. There will be no long supply cycle or supply outage. For customers, we enjoy the advantage of trial equipment. Export advantages: Desheng is a professional manufacturer of biological buffers, with strong logistics capabilities and rich export experience. We export to more than 100 countries and have established a long-term cooperation intention. HEPES packaging advantages: Only after using a good product can you know whether it is a good product in your mind. Desheng is not a company that only manufactures products once. What we want is the trust and support of our customers. We recommend that you use our trial pack first when purchasing the product. Because we firmly believe that quality is the greatest sincerity to give back to customers.
Introduction: 4-hydroxyethyl piperazine ethosulfonic acid (HEPES) is an important hydrogen ion buffer, which has a good buffer ability in the pH range of 6.8 to 8.2, and can control a constant pH for a long time. Using a concentration of 10 ~ 50mmol/L, the general culture medium containing 20mmol/L 4-hydroxyethyl piperazine taurine can have a good buffer capacity, and has no toxic effect on cells. Application: HEPES (4-hydroxyethyl piperazinethesulfonic acid) is a non-ionic amphoteric buffer with high polarity, and is inert to a variety of chemical reagents and enzymes. It does not participate in and interfere with the process of biochemical reactions, and has no inhibitory effect on enzyme chemical reactions. Therefore, it can be used specifically for the research of proteins and enzymes that are highly volatile and ph-sensitive to organelles and very volatile. Buffer solution preparation: (1)hepes buffer can be directly added to the prepared culture medium according to the desired concentration, and then filtered to remove bacteria. 2.38 g HEPES was added to each 1000ml of culture medium, the pH was adjusted to 7.2 with lNNaOH after dissolution, and the bacteria were removed by filtration before use. At this time, the concentration of HEPES was 10mmol/L. (2) 100x storage solution (lmol/L) could also be prepared. Before use, 99ml culture solution was added to lml storage solution, and the final concentration was 10mmol/L. lmol/L (100x) hepes buffer was prepared as follows: 23.8gHEPES was dissolved in 90ml double distilled water, the pH was adjusted to 7.5-8.0 with lNNaOH, and then the water was fixed to 100ml, filtered to remove bacteria, divided into vials (2ml/ bottle), and stored at 4�ºC or -20�ºC. Purpose: 1.Biological buffer; Reaction buffer, prehybridization buffer and hybridization buffer for isolation and analysis of RNA nuclear fraction; For RNA with T4RNA 2.Molecular biology grade is used for RNA3' -end labeling with T4 RNA ligase 3.Cell-cell adhesion, short-term cell aggregation culture, cleaning of tissue and cell buffer
HEPES (4-hydroxyethyl piperazine ethanesulfonic acid) is a kind of hydrogen ion buffer with pH value ranging from 6.8 to 8.2. In the process of cell culture, we often need HEPES buffer. It is a kind of weak acid. In the open culture condition, if HEPES is added, it can prevent the increase of pH oxidation in the culture medium and maintain the pH at about 7.0. The final concentration of HEPES buffer was 10-50 mmol / L, which had no toxic effect on cells. Generally, 20 mmol / L HEPES can achieve buffering capacity. Since we often use it in cell culture medium, what are its advantages in cell culture medium? Most of the cells need pH 7.2-7.4, but the optimal pH for cell culture varies with different cell types. Fibroblasts prefer higher pH (7.4-7.7), while the subculture and transformation cell lines need acid pH (7.0-7.4). HEPES is a kind of nonionic amphoteric buffer, which has good buffer capacity in the range of pH 7.2-7.4. The advantage of this method is that it can maintain a relatively constant pH value in open culture or cell observation. Under this condition, the cover of the cell culture bottle should be tightened to prevent a small amount of carbonate needed in the culture medium from scattering into the air. HEPES produced by Desheng is white crystal powder, with main content of 99.50%, iron ion 5ppm, sulfate 0.05%, and heavy metal residue (PB) 10ppm. Can provide trial sample powder, self-made solution for testing.
Buffer solution is a type of buffer system dedicated to life science research. In biochemical experiments, buffer solution plays an indispensable role. It can resist the influence of a small amount of strong acid and alkali from the outside, and maintain the system closest to the physiological environment. pH value. Both HEPES and PIPES buffers are commonly used buffers in biological experiments. The pH buffer range of HEPES is 6.8-8.2. It is a zwitterionic biological buffer. It is soluble in water and does not form stable complexes with metal ions. In most cases, it will not interfere with biochemical processes and can be widely used in a variety of Biochemical reactions and used as buffer reagents in certain cell culture media. HEPES is often used in the cell culture medium of various types of organisms; in protein research, PIPES is often used as the component and eluent of the binding buffer in cation exchange chromatography; in DNA research, PIPES is used as calcium phosphate and DNA The buffer for the precipitation formation system, AFM and the buffer for electroporation experiments. In addition, HEPES interferes with the reaction between DNA and restriction enzymes, and is not suitable for Lowry's method to determine protein content. HEPES buffer is often used in the research of organelles and highly variable, pH-sensitive proteins and enzymes, as well as in biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits. It is a hydrogen ion buffer that can control a constant pH range for a long time. The final concentration is 10-50mmol/L, and the buffering capacity can be achieved when the culture medium contains 20mmol/L HEPES. The pH buffer range of PIPES is 6.1-7.5, insoluble in water, soluble in NaOH aqueous solution. PIPES is different from buffers containing two (2-hydroxyethyl) amino groups (such as Bis-tris, Bicine), and can not form stable complexes with most metal ions. It is suitable for buffers in solution systems containing metal ions. According to existing research results, PIPES can be used to purify tubulin using phosphocellulose chromatography, to purify recombinant GTP-binding proteins ARF1 and ARF2 by gel filtration, and to use as a buffer to crystallize transketolase from E. coli. In addition, because PIPES can form free radicals, it is not suitable for redox systems. In cation exchange chromatography, a low concentration of PIPES buffer should be used, because PIPES has a relatively large ionic strength and its pKa value is concentration-dependent. We must learn to distinguish between the similarities and differences in these reagents, so as to better promote the development and production of our different products. Desheng unanimously adheres to this kind of subtle difference, so that it can continue to develop and produce better products.
The chemical name of the biological buffer HEPES product is: 4-hydroxyethylpiperazine ethanesulfonic acid, a white crystalline powder. It is a weak acid and a zwitterionic buffer in organic chemicals. The decomposition ability of the substance can increase with the increase of temperature, and decrease with the decrease of temperature. However, compared with other buffers, its decomposition constant does not change much with temperature. This makes HEPES buffers more effective at low temperatures. A buffer to keep the structure and function of the enzyme well. It is widely used in many fields such as clinical laboratory diagnostic reagents, life science research, pharmacy and biotechnology. 1) The research of biological buffer HEPES on promoting the stability of foot-and-mouth disease antigen. Foot-and-mouth disease antigen is easily degraded during storage and vaccine preparation, which affects the vaccine effect. The degradation rate of the foot-and-mouth disease antigen is related to the stability of the pH value of the maintenance solution. The preparation of a maintenance solution with strong pH stability can improve the quality of the foot-and-mouth disease vaccine. 2) Preparation of a skin care product containing a biological buffer, which contains a microbial fermentation product extract, which is characterized in that it also contains a biological buffer with a mass percentage of 0.1%-3.0%. Advantages: The pH value of the product has a small drift range. Within three years after the preparation of the skin care product, the pH value can be stabilized at 6.0-8.0, which has a good protection for the microbial fermentation product extract added therein, and the microbial fermentation product extract is not Easily hydrolyzed, the anti-aging effect of the product lasts for a long time. 3) Prepare a neuron-specific enolase content detection kit, which belongs to the field of human neuron-specific enolase content detection. This detection kit has high sensitivity and a wide detection linear range, and has the advantages of simple operation, rapidity, strong specificity, good stability and high sensitivity. In addition to HEPES, the biological buffers produced by Desheng include Tris, Bicine, Caps, Mops, Taps, EPPS, etc.; they are mainly used in biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits. Customers in need can provide samples.
Other Name: HYDROXYETHYLPIPERAZINE ETHANE SULFONIC ACID INCI Name: HYDROXYETHYLPIPERAZINE ETHANE SULFONIC ACID Molecular Formula: C8H18N2O4S Molecular Weight: 238.3 Appearance: White powder Purity: 98% CAS NO.: 7365-45-9 EINECS No.: 230-907-9 Application: cosmetics and skin care products Supplier: ZHENYIBIO HYDROXYETHYLPIPERAZINE ETHANE SULFONIC ACID has a certain effect of softening cutin and assisting in whitening; in cosmetics, it is a hydrogen ion buffer, which can control a constant pH range for a long time. Hydroxyethylpiperazine ethane sulfonic acid is a very effective biological buffer, which can reform the stratum corneum and promote the metabolism of skin keratinocytes. It is an important mild exfoliating ingredient.
We offer to manufacture different types of buffer tanks, platforms.
We are a leading supplier of electroplating chemicals, industrial polishing and welding consumables, water treatment, powder coating, fertilizers. We stock and carry an extensive range of ready to use chemicals. We deal with so many different chemicals and we would like you to contact us for more information about our different chemicals and products.
Brick Size 30 x 30 x 15 cm Net Weight 4.5 Kg 5 Kg, 11 LBS (+or- 5%) Moisture Content Below 15% Ingredients 100% Organic Coir Pith EC EC
Applications: Food ingredients for bakery, beverage, confectionery industry It is used as a raising agent in biscuits, cookies, bread, cakes, farsan, jaggery & confectionery. It acts like a buffer and carbonation agent in beverages, electrolyte and rehydration solutions.
Applications: In Animal feed stuff Nutrition feed for Poultry, Layers, Cattle. Primarily used as a dairy cow feed supplement, the buffering capability of Natural Soda's pure and natural feed grade sodium bicarbonate helps stabilize rumen pH by reducing acidic conditions. Improves calcium absorption and eggshell quality of laying hens during peak production.
Formula:KAl(SO4)2 12H2O Relative Molecular Weight:474.39 CAS NO,: 7784-24-9 EINECS No.:5637-689-7 HS CODE.: 2833301000 Appearance: lump, white crystal, white powder Properties:Colorless, transparent, particle or crystalline powder, odorless, nontoxic, tastes slightly sweet and puckery, astringent, easily soluble in water, its water solution appears acid,is would lose crystal water and become white powder on heating Usage; Food industry: Buffering agents and leavening agents in food-grade products. Chemical Industry: Water Flocculant, aluminum tanning agents and after-treating agents for leather tanning in the leather industry, dyeing agents,Astringents,mordants etc. Items Specifications Potassium aluminum sulfate [AlK(SO4)2.12H2O] % 99.5 Plumbum(Pb) mg/kg 5.0 Arsenic (As) mg/kg 2.0 Fluorine (F) mg/kg 30.0 Selenium (Se) mg/kg 30.0 Moisture % 4.0
Brand :- GARDENIA Type:- Maintain Packing :- Cartoon Size:- 5Ltr Pcs :- 4
HEPES CAS:7365-45-9 English Name:4-(2-hydroxyerhyl)piperazine-1-erhanesulfonic acid Molecular Formula:C8H18N2O4S Molecular Wt:238.31 Appearance:Free flowing white powder Assay(potertiometric cal on dry substance):99.8%. Enterprise Standard. Application: it is an important PH stable reagent in biotic experiments. Appropriate PH is obtained by mixing mild acid and its conjugate base. Many biological responses happen in neutral condition (usually PH 6-8), so the buffering range is required to be in PH 6-8. In addition, the form of acid-base of buffer should not chelate with some metallic ions. Characteristics of biological buffer: 1, Strong water-solubility; 2. Compatibility of the acid-base dissociation balance; 3. Low membrance penetration rate; 4. Low metal chelating ability; 5. High chemical stability; 6. Low absorption in ultraviolet and visible region. Packing:25KG / DRUM.
Buffered (treated) coco peat, low ec coco peat, coco chips, grow bags, coco discs, specialized coir mix.
Buffered (Treated) Coco Peat Brick 11 LBS (5KG) Net Weight :4.5 Kg 5 Kg, 11 LBS (+or- 5%) Brick Size : 30 x 30 x 15 cm EC :EC
In many biological experiments, buffers are needed to maintain an effective pH value, and buffers are critical to the success or failure of the experiment. When choosing a buffer, first consider that the pH value in the experiment is consistent with the pH value range of the buffer, and consider whether the advantages and disadvantages of different buffers are suitable for your experiment. There are many types of biological buffers. In this article, we mainly discuss the preparation methods of several commonly used biological buffers. Prepare TRIS base and TRIS-HCL buffer Tris: To prepare 1 liter of 1M Tris buffer, first prepare to dissolve 121.14 grams of Tris powder in 750 mL of dH20, adjust to the desired pH value with concentrated hydrochloric acid, and fill to the final volume of 1L with dH2O. Tris HCl: To prepare 1 liter of 1M Tris HCl buffer, first dissolve 157.60 g of Tris-HCl in 750 mL of dH2O, adjust to the desired pH with 10N sodium hydroxide, and fill with dH2O to a final volume of 1L. Prepare HEPES buffer To prepare 1 liter of 1M HEPES buffer solution, first dissolve 238.3 grams of HEPES powder in 750 mL of dH2O, adjust to the desired pH with 10N sodium hydroxide, fill with dH2O to a final volume of 1L, and pass through a filter or autoclave The device is disinfected. Store the buffer at 4C. Prepare PIPES buffer To prepare 1 liter of 1M PIPES free acid buffer solution, first add 302.37 g of PIPES free acid to 600 mL of dH2O, adjust to the desired pH with 10N sodium hydroxide, and fill to the final volume of 1L with dH2O. Desheng is a professional manufacturer of biological buffers. At present, the company sells buffers such as TRIS, HEPES, PIPES, CAPS, MOPS, BICINE, etc., with sufficient supply, fast delivery, guaranteed quality, and a professional after-sales team. If you answer any questions, you can now apply for a free sample online for trial. Please feel free to contact us if you need it.
STPP is known as Sodium Tripolyphosphate. It is commonly used in the manufacture of synthetic detergents, water treatment, and other sectors such as ceramics, food and beverage, paint and varnish, etc. It is a white molecular mineral and is used as a binder, stabilizer and sequestrant. We get our STPP mineral from several regions of Rajasthan in India. STPP has several applications in the food and beverage industry. Cake mixtures, meringues, juices, hams, peas, lima beans, cured pork, sausages, and algin sweets all contain this ingredient. Within the ceramic and enamel industry, STPP mineral is used to achieve a fluid paste or a well-dispersed solution which further optimizes the manufacturing process. Sodium triphosphate is a solubilizing binder found in powder detergents, automatic dishwashing powder/tablets, and other cleaning solutions. It is particularly effective in treating water hardness by inactivating the mineral ions and keeping them securely in the solution. It permits surfactants to perform to their maximum extent by fighting water hardness. It also functions as a pH buffer and prevents soil from resettling on the garments. This makes it the most perfect mineral in the laundry detergents, dishwashing powder and in the tanning of leather. Sodium triphosphate (STP), also sodium tripolyphosphate (STPP), or tripolyphosphate (TPP), is an inorganic compound with formula Na5P3O10. Furthermore, it is also popularly used in the paper and coatings industry to remove the pitch spots. They are created by a kind of gum present in the wood pulp which can cover the whole surface and create dents on the surface of the paper. This natural mineral is magmatic in nature and has a high phosphorus concentration and very low heavy metal levels. As a result, our STPP mineral has a high purity level which enhances the performance of whatever end-product it is utilised in.
Andean Pink Salt(Sal De Maras) Pink Salt comes from an ancient ocean, trapped underground, which feeds a spring located 10,000 feet high in the Andes Mountains in Maras, Peru. The warm spring water seeps into terraced ponds located in the Sacred Valley of the Incas. The ponds, which have been hand-harvested for over 2,000 years, are now owned by individual families, who, after harvesting, load the salt on the backs of burros for the long descent into town. Peruvian Pink Sea Salt is very pale pink and has a rich, complex, minerally flavor. It contains magnesium, iron, calcium and zinc, which can help conditions such as stress, anemia and osteoporosis. The sal de Maras is known as the healthiest and most nutritious salt on the planet, and can only be found in the Andes and the Himalayas. Health benefits of this mineral powerhouse include: It can help stabilize and regulate the heartbeat, thanks to its magnesium and sodium content. Its sodium is essential for proper muscular function. Good quality salt helps minimize the effects of stress by maintaining proper melatonin, serotonin, and tryptamine levels in the brain. It helps remove cellular acidity, especially in the brain and kidneys and it helps prevent osteoporosis. It provides a buffer for blood sugar levels to help prevent diabetes or to help those who are diabetic use less insulin. It provides iodine in a natural setting, making it easier for your thyroid to absorb it and to regulate the endocrine system.
Desheng Biochemical Technology Co., Ltd. was established in 2005. After years of innovation and development, the company has now formed a product led by "in vitro diagnostic reagent raw materials", "color reagents", "biological buffers" and other products, integrating R&D, production, Sales as one of the national high-tech enterprises. And it has established an independent research and development test center and production base, which can provide customers with high-quality research products commissioned and customized services and mass production services. Desheng Biochemicalâ??s main product biological buffer is now the main series: TAP buffer, CAPS buffer, BICIEN buffer, tris buffer, etc. The application of biological buffers in the biochemical field is very extensive, and it will not participate. And the special characteristics of not interfering with the biochemical process, the first buffer system used in life science research, to provide a stable PH experimental environment for research. In addition, Desheng Biochemical actively promotes the promotion and use of biological buffers in more fields. It is currently widely used in in vitro diagnostics, biopharmaceuticals, biological cosmetics, coatings and paints and other industries, and has reached agreements with many Fortune 500 companies. Stable cooperative relationship. Desheng biological buffer The biological buffer system contains 20-30 varieties. Desheng Technology covers several of the most important products, which can meet the routine needs of major manufacturers, whether it is the HEPES biological buffer added to the inactivated virus preservation solution, there are To help increase the survival time and infection stability of the virus, it is the commonly used Tris in DNA electrophoresis buffer, or the most widely used CAPS in Western blotting. Not to mention Bicine, it is also widely used and can be used in enzyme reaction buffer. Electrophoresis buffer, used in the preparation of stable substrate solutions, is also commonly used in serum guaninase determination. It can be said that the application of biological buffers involves all aspects. Material science needs precipitation. As a domestic company that independently develops biological buffers, Desheng has been focusing on R&D and innovation for more than ten years. With the vision of "serving for human health", it has continuously promoted the technological improvement and innovation of medical chemical materials. The field of buffers already possesses the technical strength to directly compete with international giants and has huge potential for domestic substitution. Desheng will further promote and consolidate the advantages of biological buffers in life sciences, in vitro diagnostics and other industries. Looking forward to Desheng achieving greater breakthroughs and development in the future.
