Polylysine

Introduction:
A-Polylysine, a kind of peptide with a linkage between a£­carboxyl group and examine group, is a natural substance from microbial metabolism. It has lots of advantages over traditional preservatives, such as high safety good heat tolerance and solubility in water and extensive antimicrobial against Gram+, Gram- bacteria and fungi. Now it has been applied extensively in food and pharmaceutical industry for the wide range of antimicrobial spectrum.

Physical properties:
A-Polylysine is the polymer of L-lysine. It is slightly bitter, hygroscopic, light yellow power. It is soluble in water, slightly soluble in ethanol. The 30-unit homopolymer has a molecular weight of 4700 and an empirical formula of C180H362N60O31. It is still stable when heated (12020min or 100,30min). It inhibits the thermophilics. So it is also applied to high temperature process. The activity of A-Polylysine decreases at the presence of acidic polysaccharide, chloride, phosphate, and Cu2+ while its activity increases with presence of hydrochloric acid, citric acid, malic acid, glycine and glyceride.


Safety:
A-Polylysine is recognized as a very safe substance in the scientific community. A-Polylysine was generally recognized as safe as food preservative by FDA in October, 2003.It had been approved by FDA for use as an antimicrobial agent in cooked rice or sushi rice at levels up to 50 milligrams per kilogram (mg/kg) of rice. The results of an acute toxicity study and bacterial assays for mutagenicity shows that A-Polylysine was non-toxic and there was no evidence for mutagenic activity of A-Polylysine. So A-Polylysine is absolutely safe to be used as food preservative.

Quality Standard:
FAO/WHO 1992
1. Content 95
2. Loss on drying 1053h 20
3. Heavy metalCalculated as Pb 20ppm
4. Loss on ignition 1%

Application:
A-Polylysine has already been used generally as a food additive recognized its safety and advantage in Japan and Republic of Korea. Moreover, A-Polylysine is acknowledged as a world standard food additive by this recognition of A-Polylysine as a GRAS material of FDA in US. A-Polylysine has a function to prevent a microbe from proliferating by ionic adsorption in the microbe. By such excellent properties as various antibacterial properties and properties not to destroy flavor, polylysine has been used mainly as what improves in keeping quality of food and applied to a box lunch and prepared foodstuffs in a convenience store or the like. It can be used in rice, noodles, cakes, snacks, and ice creams, salad, canned foods and meat products.
In addition, A-polylysine is used in SAP to produce sanitation products, such as sanitation napkin and diapers because of its good absorption of liquid. It can also be used as a carrier for pharmaceutical molecules. Moreover, even in a field other than food, taking advantages of characteristics of polylysine, the application to fiber, processed goods, and cosmetics as an antibacterial agent excellent in kitchen and bath room application has also been developed.
Storage:
Be laid in dry, shady and cool place with sealed packing.




0.5kg, 1kg,5kg or packed to customers requirements.

Natamycin (Pimaricin) is an antimyotic product which have broad and effective power. It is reliable in application and safe to human body. Natamycin has no effect on bacteria,so it does not prevent in the natural maturing process of food. It has been globally used in cheese, juice, fresh Ham, wine and meat industry.

Registered Index: CAS 7681-93-8, INS 235, EEC.235

Structural Formula:
Molecular formular: C33H47NO13
Molecular weight:665.75
USP 30

Items specifications of quality
Appearance White to yellow crystalline powder
Content Natamycin 50% 95%
Moisture 6.0-9.0%
pH 5.0-7.5
Ash 0.5%
Solution Color 0.07
Total plate counts Absent
Total heavy metals(as Pb) 10 ppm
Pb 3 ppm
As 1 ppm
Hg 1 ppm

Toxicity:
1. ADI 00.3mg/kg (FAO/WHO, 1994)
2. Safe to apply on food (FDA, ¡ì172.155, 1994
3. LD50 2.73g/kg (experiment with matured male mouse)

Usage:
Spraying on the surface of cheese, meat food, meat soup, ham, Guangdong flavor moon cakes, fresh juice, easily-moulded food and food processing devices to inhibit the growth of mould and yeast.

1. Cheese:
Inhibit the growing of adverse mould and promote the growing and metabolization of functioning fungus.
2. Meat:
Spraying the mixed suspension fluid on the surface of meat to effectively avoid the moulding, especially to dried sausage.
3. Baked food:
Spraying on the surface of moon cakes, pastries and bread to effectively avoid moulding, extend their shelf-life and maintain the original taste of food. Particularly for Guangdong-flavor moon cake, the egg yellow filling shall be digged into the liquid for about 2 mininutes.
4. Drinks:
Adding some Natamycin drops to fruit juice can kill the mould and yeast it contained without influencing the original taste of juice and effectively avoid juice from getting sour. The shelf life of juice can be extended. Several to dozens of ppms can achieve an ideal effect.
5. Fruit:
Spraying on the surface of apples, pears, oranges, strawberry, cherry and chestnut to prevent quality deterioration.
6. Miscellaneous:
Prevent molding of jam, jelly, marinated food, fish, chicken, man-made butter.



0.1kg, 0.5kg, 5kg, 10kg, 20kg, or on customers demand.

Nisin (also known as Streptococcus lactic peptide) is a polypeptide, with 34 amino acid residues widely used as a food preservative. Nisin possesses anti-microbial activity against a wide range of Gram-positive bacteria and their spores in food. It is catabolized to amino acid in human body, so it is no harm or vice-effect to people. Extensive micro-biological tests have not shown any cross resistant between Nisin and medical antibacterial drug. It is a natural food preservative which is highly efficient, safe and has no side-effects. In addition, it has excellent solubility and stability in food.

Character: Nisin is Grayish white or yellowish powder with hydrous potency 1000IU/mg. It is stable in room temperature and acidity condition even heated. It can endurance 30 min in pH=2.0, 121 and 15 min in pH=3.0, 121 . But at the high pH, the stability of activity can be greatly affected.

Molecular Formula: C143H228N42O37S7
Molecular Weight: 3348

ITEMS SPECIFICATIONS OF QUALITY
Appearance Grayish white or yellowish powder
Content (IU/mg): 1000
Moisture Content: 3%
pH of 10% Aqueous Solution 3.10-3.60
Pb 1mg/kg
As 1mg/kg
Sodium Chloride : 50%
Hydrous Potency : 1 10 6 IU/g
Microbiological Count 10cfu/g
E.Coli in 25gAbsent
Salmonella in 25g Absent

Amount for Application:
GB 2760-90;
FAO/WHO, 1984;
FDA 184.1;
E234
EINECS215-807-5

Toxicity: GRAS(FDA 184.1538, 1994); ADI 33000 IU/kg (FAO/WHO, 1994)

Usage:
1.Fresh milk and dairy products:
Adding 0.05g/kg nisin to fresh milk, it can control bacterial spoilage and extend the shelf life to 2-3 times. Revealed by American experiments, adding nisin to reduced fat milk, no salt butter, degrease milk and flavored milk, it can extend the shelf life to six weeks even at 45 .
2.Liquid egg and egg products:
Adding 0.05-0.1g/kg nisin can efficiently inhibit bacillus spp and extend the shelf life from seven days to one month.
3.Salad and sauce:
Adding nisin to salad or sauce, it can efficiently control the growth of lactococcus lactis and spore and extend the shelf life to four times. The recommended amount is 0.05-0.2g/kg.
4.Canned foods:
Showed by many research, adding nisin to canned vegetable, it can control bacterial spoilage and shorten the time of heat processing required; adding nisin to canned rice wine, it can reduce 90% time of the heat processing, enhance product quality; adding nisin to canned bean, the shelf life can be extent to two years even at 55 .
5.Cured meat/sea foods
The shelf life extend to 2-3 times when using nisin. We can spray nisin liquid on the surface of the products or add nisin into products with the dosage of 0.1-0.2g/kg.
6.Canned soup/juice and drink
Adding 0.1-0.2g/kg can efficiently inhibit the growth of bacteria spores.


0.1kg, 0.5kg, 5kg, 10kg, 20kg, or on customers demand.

Characters:
Ivory powder or granular solid, it can be dispersed into the hot water, can be dissolved into the ethanol and hot grease. It is a non-ionic emulsifier, HLB value is 3.8-5.3.
Character:
1. It is good for form stable emulsion to avoid delamination and deposit of food and beverage by adding emulsifier. And it could improve quality of product and prolong shelf life of product
2. It can form the complex with the protein and starch, prevent the starch from aging, used in bread, cake for keeping the shelf life.
3. It can be arranged in order on surface of grease. This is helpful to control and stabilize crystallization of grease. Especially in production of margarine, it can improve plasticity and malleability of product to avoid delamination of the oil and water.