Tris Buffer

The Chinese name of Tris is tris(hydroxymethyl)aminomethane, tromethamine, tromethamine, 2-amino-2-(hydroxymethyl)-1,3-propanediol. It is a white crystal or powder. Soluble in ethanol and water, slightly soluble in ethyl acetate and benzene, insoluble in ether and carbon tetrachloride, corrosive to copper and aluminum, and irritating chemicals. Tris has a high buffer capacity, high solubility in water, and is inert to many enzymatic reactions, making Tris a very satisfactory buffer for many biochemical purposes. It is generally used to stabilize the pH of the reaction system, and has a strong buffering capacity between pH 7.5-9.0. Advantages of Tris buffer: 1. Because Tris base is more alkaline, you can only use this buffer system to configure a buffer with a wide range of pH ranging from acidic to alkaline; 2. It has little interference to biochemical processes, and does not precipitate with calcium, magnesium ions and heavy metal ions. Disadvantages of Tris buffer: 1. The pH of the buffer is greatly affected by the concentration of the solution, the buffer is diluted ten times, and the pH change is greater than 0.1; 2. The temperature effect is large, and the temperature change has a great influence on the pH of the buffer. The pH of the buffer at 4�?�°C is 8.4, and the pH at 37�?�°C is 7.4. It must be prepared at the temperature of use, and prepared at room temperature Tris-HCL buffer cannot be used for 0-4; 3. It is easy to absorb CO2 in the air, so the prepared buffer should be tightly sealed; 4. This buffer has a certain interference effect on some PH electrodes, so use electrodes that are compatible with Tris solution. Application of Tris buffer: It forms a buffer system with glycine in the electrophoresis buffer to stabilize the pH; uses the Tris-HCL buffer system in the gel to stabilize the pH; it is widely used as a solvent for nucleic acids and proteins; the low ionic strength of the Tris buffer can also be used The formation of intermediate fibers of nematodes; add EDTA to Tris hydrochloric acid buffer to make "TE buffer", which can be used for DNA stabilization and storage; change the pH-adjusting acid solution to acetic acid to obtain "TAE buffer", change "TBE buffer" can be obtained as boric acid. These two solutions are used in nucleic acid electrophoresis experiments.

Tris, or tris(hydroxymethyl)aminomethane, has been widely used as a pH buffer in biological media for about 35 years. The near-ideal properties of this biological buffer are the reason for its popularity. Tris does not absorb water, is easily soluble in water, and has high purity. It does not precipitate calcium salts, is stable in solution for several months at room temperature, and does not seem to inhibit many enzyme systems. However, due to the chemical and physical properties of Tris buffer, improper use may lead to erroneous pH measurement of the electrode system. The influence of pH in TRIS buffer on electrode Biochemists use TRIS buffers to control the pH within the physiological range (approximately 7 to 8 pH), because phosphate can cause adverse reactions with biological substances in the test sample. However, when pH measurements are to be made on these solutions, another type of "adverse side reaction" must be recognized, involving the pH electrode system. The influence of chemical and physical properties of Tris buffer on pH Tris, which is commonly used for physiological measurement (pH 7 to 7.5), does not have a large buffer capacity. Therefore, caution should be exercised when using tris buffer to control sample pH. The rather large temperature coefficient of tris (-0.028pH/ C) is also worth considering. For example, if the meter is calibrated with tris buffer and the tris temperature is 20 C, using a pH value of 25 C will cause errors. Therefore, temperature pH dependence must be used when using tris for standardization. When using tris to control the pH of the sample, the degree of control will depend on the sample temperature. If the sample temperature changes by 2 C, the pH control changes by about 0.06 pH units. The required pH control will determine the maximum allowable temperature fluctuations. Advantages of Tris buffer Because the Tris base is highly alkaline, this buffer system can be used to prepare buffers with a wide range of pH values from acidic to alkaline; It has little interference to the biochemical process, and does not precipitate with calcium, magnesium ions and heavy metal ions. Disadvantages of Tris buffer The pH value of the buffer is greatly affected by the concentration of the solution. The buffer is diluted ten times, and the pH change is greater than 0.1; the Tris buffer should be prepared from high-purity tris and HCl using distilled or deionized water without CO2. The temperature effect is large, and the temperature change has a great influence on the pH value of the buffer, so it must be prepared at the use temperature, and the Tris-HCl buffer prepared at room temperature cannot be used at 0 C to 4 C; It is easy to absorb CO2 in the air, so the prepared buffer should be tightly sealed; This buffer has a certain interference effect on some pH electrodes, so use an electrode compatible with Tris solution.

Chinese Name: bis (2-hydroxyethyl) amino (trihydroxymethyl) methane English Name: 2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol CAS No.: 6976-37-0 Molecular formula: C8H19NO5 Molecular weight: 209.24 Storage conditions: Store at RT Purpose: It can be used in biological buffer for detecting creatine kinase. It is also used to protect hemoglobin in freeze drying. Cathodic electrolyte is used in IEF/2-D gel electrophoresis.

Biological buffer Title: tris (hydroxymethyl) aminomethane Tris English name Trometamol Chinese name Tris-( hydroxymethyl)-aminometh

In many biological experiments, buffers are needed to maintain an effective pH value, and buffers are critical to the success or failure of the experiment. When choosing a buffer, first consider that the pH value in the experiment is consistent with the pH value range of the buffer, and consider whether the advantages and disadvantages of different buffers are suitable for your experiment. There are many types of biological buffers. In this article, we mainly discuss the preparation methods of several commonly used biological buffers. Prepare TRIS base and TRIS-HCL buffer Tris: To prepare 1 liter of 1M Tris buffer, first prepare to dissolve 121.14 grams of Tris powder in 750 mL of dH20, adjust to the desired pH value with concentrated hydrochloric acid, and fill to the final volume of 1L with dH2O. Tris HCl: To prepare 1 liter of 1M Tris HCl buffer, first dissolve 157.60 g of Tris-HCl in 750 mL of dH2O, adjust to the desired pH with 10N sodium hydroxide, and fill with dH2O to a final volume of 1L. Prepare HEPES buffer To prepare 1 liter of 1M HEPES buffer solution, first dissolve 238.3 grams of HEPES powder in 750 mL of dH2O, adjust to the desired pH with 10N sodium hydroxide, fill with dH2O to a final volume of 1L, and pass through a filter or autoclave The device is disinfected. Store the buffer at 4C. Prepare PIPES buffer To prepare 1 liter of 1M PIPES free acid buffer solution, first add 302.37 g of PIPES free acid to 600 mL of dH2O, adjust to the desired pH with 10N sodium hydroxide, and fill to the final volume of 1L with dH2O. Desheng is a professional manufacturer of biological buffers. At present, the company sells buffers such as TRIS, HEPES, PIPES, CAPS, MOPS, BICINE, etc., with sufficient supply, fast delivery, guaranteed quality, and a professional after-sales team. If you answer any questions, you can now apply for a free sample online for trial. Please feel free to contact us if you need it.

White, crystalline, hygroscopic powder CAS : 6850-28-8 Product ID : TRISAC Molecular formula : C6H15NO5 Molecular mass : 181.19g/mol MP: 115-120C Flash Point: 165 - 173C Solubility: 80 - 100g/L at 20C Tris acetate is a white, crystalline, powder with a faint vinegar odor. Tris acetate, or tris(hydroxymethyl) aminomethane acetic acid salt, is a is highly soluble ammonium salt commonly used as a biological buffer ingredient biological, medical, and biochemical processes. One such process requiring pH control with tris acetate is the DNA extraction. DNA extraction is a pH-sensitive process which uses tris acetate buffer to keep the pH stable between pH 7 and 9. Tris-acetate is also used to prepare TAE buffer (tris-acetate-EDTA), which is used as a running buffer for DNA agarose gels.

Every biological system has a well-known buffer system to maintain the acid-base balance within a specific pH range. Our lives depend on the function of the buffer system. A buffer system is a solution that resists changes in pH when acid or alkali is added. Different buffers have different ability and efficacy for skin replacement. The high-quality Good's Buffers produced by Desheng are usually used in cosmetics, such as toners, essences, lotions, eye creams and facial masks, etc., according to your application. Here are 4 biological buffers commonly used in cosmetics. 1) HEPES (4-hydroxyethylpiperazine ethanesulfonic acid) CAS Number: 7365-45-9 Useful pH range: 6.8-8.2 The main purpose 1. Acidity regulator, or pH control agent. 2. An effective concentration of HEPES can provide an exfoliating effect. 3. Promote the transdermal absorption of various functional ingredients in cosmetics. 2) TRIS (Tris(hydroxymethylaminomethane)) CAS number: 77-86-1 Useful pH range: 7.2-9.0 use: 1. Non-ionic surfactants-amines 2. Acidity regulator or pH control agent. 3. Excellent cleaning ability and emulsifying ability. 3) BIS-TRIS (Trimethylol) Methylamino Propane) CAS Number: 5625-37-6 Useful pH range: 6.1-7.5 The main purpose 1. Non-ionic surfactants-amines 2. Acidity regulator, or pH control agent. 3. Excellent decontamination, dispersion, emulsification and antistatic ability. 4) Glycylglycine buffer (G-132) CAS Number: 7365-44-8 Useful pH range: 7.5-8.9 The main purpose: 1. Adjust the pH range of the solution 2. Condition the skin, reduce pores and prevent skin aging. 3. Excellent emulsification, dispersion, antistatic, wetting, penetration, and decontamination capabilities Precautions External factors, water and detergents may reduce the local buffering capacity, because the elution of buffer chemicals leads to increased pH and irritant contact dermatitis. Inflammatory diseases, including atopic dermatitis, psoriasis, and acne vulgaris, increase in pH and decrease buffering capacity. For the treatment of skin diseases and aging skin, emollients with a pH value slightly higher than the average normal pH value and with appropriate buffering capacity should be used first. Desheng is a professional manufacturer of biological buffers. There are many cooperative manufacturers in the skin care industry. In addition to the two biological buffers Hepes and Tris, it also produces many other models such as Mops, Taps, Pipes, Caps, and the core product tris buffer. Known for its ability to maintain the original color of emulsions and excellent odor suppression capabilities, it is widely used in cosmetics such as skin care lotions, sunscreens, eye makeup products, nail polish, etc. It can maintain the functional effectiveness of active substances and is a skin care product The industry's trusted choice.

1. Tris brief introduction As the handling in biological buffers, tris plays a more important role, not only because of the wide pH range of tris, it can act as a pH regulator. Conventionally, it is white crystal particles, but there are certain requirements for water solubility, and white powder will not meet its requirements better. The pH value of the acid-base indicator is 7-9, and there are other names, tromethamine, ammonium tromethamine, trishydroxymethylaminomethane and so on. Although they are all the same product, different buyers will call them differently based on different fields. Second, the use of Tris 2. Tris application in cosmetics With the improvement of peoples quality of life, more and more attention is paid to spiritual life. Among them, the heart of beauty is common to everyone, regardless of men and women: appearance has become an important indicator of first impression (of course I strongly disagree Because I dont have that beauty. The rise and mass use of cosmetics have become inevitable. To make good cosmetics, the ingredients are very important. The role of Tris in cosmetics is basically to adjust the pH, and it can also be used as a neutralizer. The PH value of healthy Asian skin is slightly acidic, and the ingredients in many cosmetics are very complicated. If you want to adjust the pH value, you must use tris to adjust, so that the entire raw material composition will not change too much. Many people see that tromethamine is a chemical product, and it is very scary to use in cosmetics, and they are afraid of being unsafe. In fact, if the proportion of ingredients is used properly, there is no need to worry about it. Generally, it will not harm the skin. 3.Tris is used for synthesis Tris with a relatively simple structure can synthesize many products, and the same buffer system, Tris-HCL, Bis-Tris Tricine, TES, TAPS, etc. can all be synthesized with tris. Compared with others, the price of Tris will be cheaper, but the synthesized products can be applied to more fields, and the price will be doubled. Therefore, the synthesis of tris can be said to be a hot pastry.

De Sheng is a professional blood testing reagent company. It is a single enterprise with a complete industrial chain in China. Up to now, it has more than 10 years of R&D and production experience. Biobuffer products developed by Liu technical team of the company include Tris, Bicine, Caps, Mops, Taps, EPPS, HEPES, MOPSO, PIPES and so on, which are well received. DeSheng brand Tri (hydroxymethyl) Angelica Tris introduced: Trometamol Name Trimethylol Angelica End Tris CAS# 77-86-1 Molecular Weight]121.135 Molecular Formula Adidine H11NO3 Storage Conditions Room temperature, light-proof and moisture-proof Appearance White Crystal Powder PH Scope] 7-9 Melting Point 168-172 degrees C 1. Dissolve Tris with a small amount of double-steamed water (300-500 ml). After adding HCl, adjust the pH to 7.6 with HCl (1N) or NaOH (1N), and add extra-thick double-steamed water to 1000 ml. This liquid is a reserve liquid, which is stored in refrigerator at 4 C. Uses: Buffer preparation in biochemical and molecular biology experiments; intermediate; preparation of surfactants and vulcanization accelerators; preparation of water-soluble polymers containing Tris structural units as paint dispersants; preparation of indoor formaldehyde adsorption materials, etc., Tris is also used as titration standard. 1. Buffering properties] Tris is a weak base, and its pKa is 8.1 at room temperature (25 C); according to the buffer theory, the effective buffer range of Tris buffer is between pH 7.0 and 9.2. Desheng products include good's buffer: Bicine, Caps, EPPS, MOPS, TAPS, HEPES, MOPSO, PIPES; blood vessel extracting additives: serum separating gel, sodium glycerin, glycerin, EDTA dipotassium, EDTA tripotassium, coagulant, planning agent, sodium citrate, planning potassium; chromogenic substrates: TOOS, TOPS, ADOS, ADP S, ALPS, DAOS, HDAOS, MADB, MAOS, PEP, etc. Luminescent reagents: Azidine ester, Luminol, Azidine salt and isoluminol. All in vitro diagnostic reagents are purified by HPLC analysis method and the purity is more than 99%. As the manufacturer develops and sells, the DeSheng color substrates are in stock and the quality is guaranteed. Enterprise friends who need color raw materials can contact Desheng, address C8, Guanggu United Science and Technology City, Ezhou, Hubei Province, and look forward to your visit!