PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible PCR.
Highlights
Convenient, ready-to-use mix
Thermostablehalf life is more than 40 min at 95°C
Generates PCR products with 3'-dA overhangs
Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)
Applications
Routine PCR amplification of DNA fragments up to 5 kb
High throughput PCR
DNA labeling
Taqman Master Mix is designed for high throughput quantitative PCR using Taqman probe-based chemistry. Available with the option of ROX or fluorescein as the internal passive reference dye, Taqman 2X qPCR Master Mix offers superb performance in sensitivity and signal-to-noise ratio. The formulation supports quantitative amplification and detection of up to four targets simultaneously with consistent and reliable results.
