Ed. If only one red line appears, it indicates that the cow is not Pregnant, p4 is in the lowest level at this moment. It is recommended to make the best use of this time to Because the diary cow¡¯s estrous cycle is 18-22 days, it is suggested to perform the test the 18th days after . In order to get reliable result, give up the first three Milking and then collect sample milk around 5-10ml. Mix the sample milk well before testing. Urine and blood Positive: if both t band and c band are visible, the test result is positive, which means the cow is pregnant or In middle period of estrous cycle. Negative: if only c line is visible, the test result is negative, which indicates the cow is not pregnant. Invalid: when control line does not appear on the membrane, the test is invalid due to improper test Procedure or deterioration of reagents. It is recommended that the test be repeated. Precaution (1) test according to the law of the estrous cycle strictly. (2) for single use only; do not reuse the test devices. (3) don¡¯t keep the strip or cassette in the air for a long time after unpacking. (4) while performing the test, make sure that the surface of sample should not exceed the ¡°max¡± line of strip. (5) do not use test kit after expiry date. Validity and storage The period of the validity is 24 months. Store as packaged in the sealed pouch under the room temperature. Avoid light. In house standard