European larvae rancidosis, referred to as larval disease, is a bacterial digestive tract disease of bee larvae, which occurs all over the world and has the characteristics of rapid spread and rapid onset. The antibacterial substance of HK-1 strain has strong antagonistic effect on common bee bacterial diseases such as H. mellifera, Hive Hafenia, Bacillus larvae, and bee bulb, and the fermentation trait is excellent. The macroporous adsorbent resin provided by Suzhou Bojie Resin Technology Co., Ltd. can effectively enrich the antibacterial substance of HK-1 strain, which has large adsorption capacity, high adsorption rate, fast adsorption rate, no other impurities in the adsorption process, and strong selectivity. The desorption is simple, and can be repeatedly used , with high recycling rate, wear-resistant , long service life, and also economical and effective.