STANDARD M nCoV Real-Time Detection kit is used for rapid identification and detection of novel coronavirus (2019-nCoV) nucleic acids in human nasopharyngeal swabs and throat swab samples. Coronavirus RNA was first transcribed into cDNA by reverse transcriptase, and then cDNA was used as a template for PCR amplification. The separation of the fluorophore and quencher makes the fluorescence signal detected by the instrument: FAM channel qualitative detection of the new coronavirus (COVID-19) ORF1ab gene, and JOE channel qualitative detection of coronavirus) E gene, and CY5 channel detection internal reference. The kit uses dUTP and UNG enzymes to prevent contamination of amplification products.